Gauting, Germany
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Author: IMLred

Institute of Microbiology and Laboratory Medicine - Department of Research, Education, and Development

Capacity of Abbott RealTime MTB RIF/INH to detect rifampicin- and isoniazid-resistant tuberculosis

Hofmann-Thiel, S., Molodtsov, N., Duffner, C., Kadyrov, A., Kalmambetova, G., Kabirov, O.5; Rajabov, A., Parpieva, N., Sayfutdinov, Z., Vogel, M., Vogel, H., Antonenka, U., Hoffmann, H. Abstract BACKGROUND: Abbott RealTime MTB RIF/INH Resistance (RT RIF/INH) is a new assay for the detection of resistance to rifampicin (RIF) and isoniazid (INH) in tuberculosis (TB) patients. OBJECTIVE: To evaluate the capacity of RT RIF/INH to detect resistance-associated mutations in target genes. METHODS: A…
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Equal sensitivity of the new generation QuantiFERON-TB Gold plus in direct comparison with the previous test version QuantiFERON-TB Gold IT

H.Hoffmann, K.Avsar, R.Göres, S.-C.Mavi, S.Hofmann-Thiel Abstract QuantiFERON-TB Gold IT analyses interferon-γ release from CD4+ T cells after stimulation with specific tuberculosis (TB) antigens. Its sensitivity is approximately 80% for active TB. A new test generation (QFTGplus) also analyses the response of CD8+ T cells. We investigated both test generations in a direct head-to-head comparison in a German pulmonary hospital. Sensitivity rates for active…
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Evaluation of the Abbott RealTime MTB and RealTime MTB INH/RIF Assays for Direct Detection of Mycobacterium tuberculosis Complex and Resistance Markers in Respiratory and Extrapulmonary Specimens

Sabine Hofmann-Thiel, Nikolay Molodtsov, Uladzimir Antonenka, Harald Hoffmann ABSTRACT The Abbott RealTime MTB (RT MTB) assay is a new automated nucleic acid amplification test for the detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. In combination with the RealTime MTB INH/RIF (RT MTB INH/RIF) resistance assay, which can be applied to RT MTB-positive specimens as an add-on assay, the…
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How should discordance between molecular and growth-based assays for rifampicin resistance be investigated?

Hofmann-Thiel, S., Hoffmann, H., Hillemann, D., Rigouts, L., Van Deun, A., Kranzer, K. Abstract Molecular tests to detect the presence of Mycobacterium tuberculosis and genetic polymorphisms in the rpoB gene conferring resistance to rifampicin (RMP) have become integral parts of tuberculosis diagnostics worldwide. These assays are often performed sequentially or in parallel to phenotypic drug susceptibility testing. Discordances between molecular and phenotypic tests…
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Evaluation of Fluorotype MTB for detection of Mycobacterium tuberculosis complex DNA in clinical specimens from a low-incidence country

Sabine Hofmann-Thiel & Harald Hoffmann  Abstract Background With Fluorotype MTB (FT MTB, HAIN Lifesciences, Germany) a new semi-automated assay for detection of M. tuberculosis complex (MTBC) in clinical specimens has been introduced. In a prospective study, we evaluated the diagnostic performance of FT MTB in a routine diagnostic setting in a low-incidence country. Methods A total of 1039 respiratory…
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Comparison of Xpert MTB/RIF with ProbeTec ET DTB and COBAS TaqMan MTB for direct detection of M. tuberculosis complex in respiratory specimens

Uladzimir Antonenka, Sabine Hofmann-Thiel, Laziz Turaev, Ainura Esenalieva, Mokhonim Abdulloeva, Evgeni Sahalchyk, Tarig Alnour & Harald Hoffmann  Abstract Background Nucleic acid amplification assays allow for the rapid and accurate detection of Mycobacterium tuberculosis (MTB) directly in clinical specimens thereby facilitating diagnosis of tuberculosis (TB). With the fully automated Xpert MTB/RIF system (Cepheid) an innovative solution of TB diagnostics has been launched. We performed a direct…
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Multi-centre evaluation of the speed-oligo Mycobacteria assay for differentiation of Mycobacteriumspp. in clinical isolates

Sabine Hofmann-Thiel, Laziz Turaev, Tarig Alnour, Lore Drath, Maria Müllerova & Harald Hoffmann  Abstract Background A new DNA line probe assay (Speed-oligo Mycobacteria, Vircell) has been launched for rapid differentiation of Mycobacterium spp. from cultures. Compared to other line-probe assays, Speed-oligo Mycobacteria covers a relatively limited spectrum of species but uses a simpler and faster dip-stick technique. The present multi-centre, multi-country study aimed…
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Evaluation of the hyplex® TBC PCR test for detection of Mycobacterium tuberculosis complex in clinical samples

Sabine Hofmann-Thiel, Laziz Turaev & Harald Hoffmann  Abstract: Background Tuberculosis (TB) is one of the major public health concerns worldwide. The detection of the pathogen Mycobacterium tuberculosis complex (MTBC) as early as possible has a great impact on the effective control of the spread of the disease. In our study, we evaluated the hyplex® TBC PCR test (BAG Health Care…
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Mechanisms of heteroresistance to isoniazid and rifampin of Mycobacterium tuberculosis in Tashkent, Uzbekistan

S. Hofmann-Thiel, J. van Ingen, K. Feldmann, L. Turaev, G. T. Uzakova, G. Murmusaeva, D. van Soolingen, H. HoffmannEuropean Respiratory Journal 2009 33: 368-374; DOI: 10.1183/09031936.00089808 Abstract: Heteroresistance of Mycobacterium tuberculosis (MTB) is defined as the coexistence of susceptible and resistant organisms to anti-tuberculosis (TB) drugs in the same patient. Heteroresistance of MTB is considered a preliminary stage to full resistance. To date, no mechanism causing heteroresistance of MTB has been proven. Clinical specimens and cultures from…
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Equal sensitivity of the new generation QuantiFERON-TB Gold plus in direct comparison with the previous test version QuantiFERON-TB Gold IT

H.Hoffmann, K.Avsar, R.Göres, S.-C.Mavi, S.Hofmann-Thiel Abstract QuantiFERON-TB Gold IT analyses interferon-γ release from CD4+ T cells after stimulation with specific tuberculosis (TB) antigens. Its sensitivity is approximately 80% for active TB. A new test generation (QFTGplus) also analyses the response of CD8+ T cells. We investigated both test generations in a direct head-to-head comparison in a German pulmonary hospital. Sensitivity rates for active…
Read more